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Farrerol inhibits IL-6 and IL-8 production in LPS-stimulated human gingival fibroblasts by suppressing PI3K/AKT/NF-κB signaling pathway.
- Source :
-
Archives of Oral Biology . Feb2016, Vol. 62, p28-32. 5p. - Publication Year :
- 2016
-
Abstract
- Objectives Farrerol, a new type of 2,3-dihydro-flavonoid isolated from rhododendron, has been shown to have anti-bacterial and anti-inflammatory activities. In the present study, we investigated the anti-inflammatory effects of farrerol on the production of IL-6 and IL-8 in human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS). Methods The cytotoxicity of farrerol was determined using the MTT assay. The production of IL-6 and IL-8 was measured using ELISA and qRT-PCR. The effects of farrerol on PI3K, Akt phosphorylation, and NF-κB activation were detected using western blotting analyses. Results These results showed that farrerol inhibited LPS-induced IL-6 and IL-8 production in a dose dependent manner. LPS-induced NF-κB activation was suppressed by farrerol. Furthermore, farrerol suppressed LPS-induced PI3K and Akt phosphorylation, which are upstream molecules of NF-κB. Conclusion These results indicated that farrerol attenuated IL-6 and IL-8 production by inhibition of PI3K and AKT phosphorylation, resulting in an inhibition of NF-κB activation. Farrerol may be a therapeutic agent for the treatment of periodontal disease. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00039969
- Volume :
- 62
- Database :
- Academic Search Index
- Journal :
- Archives of Oral Biology
- Publication Type :
- Academic Journal
- Accession number :
- 111977326
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2015.11.007