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microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.
- Source :
-
BMC Molecular Biology . 12/29/2015, Vol. 16, p1-9. 9p. 1 Chart, 5 Graphs. - Publication Year :
- 2015
-
Abstract
- Background: Dysregulation of microRNA-150 (miR-150) is commonly observed in solid tumor and has been reported to be involved in multiple important biological processes, such as cell proliferation, apoptosis, and metastasis. Elevated miR-150 level was also detected in cervical carcinoma, whereas its function in cancer progression has not been studied yet. Methods: The expression of miRNA-150 in cervical carcinoma was compared with normal cervical tissue and using qRT-PCR. The effects of miR-150 on cell cycle and apoptosis, as well as the expression of cycle- and apoptosis-related genes, were determined using flow cytometry, TUNEL assay, qRT-PCR, and Western blot, respectively. The direct target of miR-150 was confirmed using 3' untranslated region (UTR) luciferase reporter assay. Results: miR-150 promotes cervical cancer cell survival and growth, while the inhibition of miR-150 suppresses these actions. miR-150 also induced the cell cycle progression from G1/G0 to S phase, resulting in an enhancement of growth. Several cell cycle- and apoptosis-related genes, CyclinD1, p27, BIM, and FASL were modulated by miR-150. Moreover, miR-150 directly reduced the expression of FOXO4, which regulates the expression of CyclinD1, p27, BIM, and FASL, by targeting its 3' UTR. Conclusion: Taken together, our data demonstrated that elevated miR-150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell growth and survival. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*CERVICAL cancer
*CANCER cells
*CARCINOMA
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 14712199
- Volume :
- 16
- Database :
- Academic Search Index
- Journal :
- BMC Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 112024744
- Full Text :
- https://doi.org/10.1186/s12867-015-0052-6