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LIB spectroscopic and biochemical analysis to characterize lead toxicity alleviative nature of silicon in wheat (Triticum aestivum L.) seedlings.

LIB spectroscopic and biochemical analysis to characterize lead toxicity alleviative nature of silicon in wheat (Triticum aestivum L.) seedlings.

Authors :
Tripathi, Durgesh Kumar
Singh, Vijay Pratap
Prasad, Sheo Mohan
Dubey, Nawal Kishore
Chauhan, Devendra Kumar
Rai, Awadesh Kumar
Source :
Journal of Photochemistry & Photobiology B: Biology. Jan2016, Vol. 154, p89-98. 10p.
Publication Year :
2016

Abstract

The responses of wheat seedling treated with silicon (Si; 10 μM) and lead (Pb; 100 μM) for 7 days have been investigated by analyzing growth, Pb uptake, chlorophyll fluorescence, oxidative stress, antioxidants and nutrients regulation. Results indicated that, Pb significantly (P < 0.05) declined growth of seedlings which was accompanied by uptake of Pb. Under Pb stress, fluorescence parameters: F v /F m ratio and qP were significantly (P < 0.05) decreased while NPQ was increased. Si addition alleviated Pb-induced decrease in growth and alterations in photosynthesis, and also significantly (P < 0.05) lowered Pb uptake. Under Pb treatment, oxidative stress markers: hydrogen peroxide and lipid peroxidation were enhanced while DPPH • scavenging capacity and total phenolic compounds (TPCs) were decreased significantly, however, Si addition improved the status of antioxidants. The non-protein thiols (NP-SH) showed enhanced level under Pb stress. Pb stress considerably disturbed status of the nutrients as decrease in Ca, P, Mg, Zn and Ni contents while an increase in K, S, B, Cu, Fe, Mn and Na contents were noticed. Si addition maintained status of all the nutrients remarkably. The quickest method of element analysis: LIBS spectra revealed significantly lower uptake of Pb in seedlings grown under Si and Pb combination and same was correlated with the data of AAS. Overall results pointed out that excess Pb uptake disturbed status of nutrients, photosynthetic performance, antioxidant capacity, hence severe oxidative damage to lipids occurred. Further, Si supplementation successfully regulated these parameters by inhibiting Pb uptake hence maintained growth of wheat seedlings. Similar pattern of data recorded by the LIBS, AAS and ICAP-AES confirmed that LIBS may be one of the promising and authentic tools to monitor the mineral and metal distribution in the plants without hampering or disturbing the environment due to its eco-friendly and non-invasive nature. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10111344
Volume :
154
Database :
Academic Search Index
Journal :
Journal of Photochemistry & Photobiology B: Biology
Publication Type :
Academic Journal
Accession number :
112052998
Full Text :
https://doi.org/10.1016/j.jphotobiol.2015.11.008