Development and validation of a direct-comparison method for cardiac I-metaiodobenzylguanidine washout rates derived from late 3-hour and 4-hour imaging.

Purpose: The washout rate (WR) has been used in I-metaiodobenzylguanidine (MIBG) imaging to evaluate cardiac sympathetic innervation. However, WR varies depending on the time between the early and late MIBG scans. Late scans are performed at either 3 or 4 hours after injection of MIBG. The aim of this study was to directly compare the WR at 3 hours (WR) with the WR at 4 hours (WR). Methods: We hypothesized that the cardiac count would reduce linearly between the 3-hour and 4-hour scans. A linear regression model for cardiac counts at two time-points was generated. We enrolled a total of 96 patients who underwent planar I-MIBG scintigraphy early (15 min) and during the late phase at both 3 and 4 hours. Patients were randomly divided into two groups: a model-creation group (group 1) and a clinical validation group (group 2). Cardiac counts at 15 minutes (count), 3 hours (count) and 4 hours (count) were measured. Cardiac count was mathematically estimated using the linear regression model from count and count. Results: In group 1, the actual cardiac count/count was highly significantly correlated with count/count ( r = 0.979). In group 2, the average estimated count was 92.8 ± 31.9, and there was no significant difference between this value and the actual count (91.9 ± 31.9). Bland-Altman analysis revealed a small bias of −0.9 with 95 % limits of agreement of −6.2 and +4.3. WR calculated using the estimated cardiac count was comparable to the actual WR (24.3 ± 9.6 % vs. 25.1 ± 9.7 %, p = ns). Bland-Altman analysis and the intraclass correlation coefficient showed that there was excellent agreement between the estimated and actual WR. Conclusion: The linear regression model that we used accurately estimated cardiac count using count and count. Moreover, WR that was mathematically calculated using the estimated count was comparable to the actual WR. [ABSTRACT FROM AUTHOR]