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Aquaporin-3 Inhibition Reduces the Growth of NSCLC Cells Induced by Hypoxia.
- Source :
-
Cellular Physiology & Biochemistry (Karger AG) . Jan2016, Vol. 38 Issue 1, p129-140. 12p. - Publication Year :
- 2016
-
Abstract
- Background/Aims: Non-small cell lung cancer (NSCLC) is the leading cause of death worldwide. Although aquaporin-3 (AQP3) is widely distributed in mammalian tissues and over-expressed in NSCLC cells, there are limited studies on the effects of AQP3 knockdown on NSCLC cells under hypoxic conditions. Methods: The CCK-8 assay was used to calculate cell viability. Scratch-wound healing and transwell assays were used to detect cell migration and invasion. Apoptotic cells were measured by the TUNEL assay. mRNA expression levels were calculated via quantitative RT-PCR. Relative protein levels were determined by immunoblot assays. Results: AQP3 knockdown substantially reduced proliferation, migration, and invasion of A549 and NCI-H460 cells under hypoxic conditions. Moreover, AQP3 knockdown clearly induced cell apoptosis. Further analysis identified levels of HIF-1α, VEGF, Raf, phosphor-MEK, and phosphor-ERK, whose activities were significantly attenuated in the AQP3 knockdown group. Conclusions: These findings indicate that AQP3 knockdown retards the growth of NSCLC cells partially through inhibiting HIF-1α/VEGF and Raf/MEK/ERK signalling pathways. © 2016 The Author(s) Published by S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- *AQUAPORINS
*NON-small-cell lung carcinoma
*HYPOXEMIA
*CAUSES of death
*CANCER cells
Subjects
Details
- Language :
- English
- ISSN :
- 10158987
- Volume :
- 38
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cellular Physiology & Biochemistry (Karger AG)
- Publication Type :
- Academic Journal
- Accession number :
- 112573673
- Full Text :
- https://doi.org/10.1159/000438615