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The Safety and Immunogenicity of an Interleukin-12-Enhanced Multiantigen DNA Vaccine Delivered by Electroporation for the Treatment of HIV-1 Infection.
- Source :
-
JAIDS: Journal of Acquired Immune Deficiency Syndromes . 2/1/2016, Vol. 71 Issue 2, p163-171. 9p. - Publication Year :
- 2016
-
Abstract
- Background: Therapeutic vaccination is being studied in eradication and "functional cure" strategies for HIV-1. The Profectus Biosciences multiantigen (MAG) HIV-1 DNA vaccine encodes HIV-1 Gag/Pol, Nef/Tat/Vif, and Envelope, and interleukin-12 (IL-12) and is delivered by electroporation combined with intramuscular injection (IM-EP). Methods: Sixty-two HIV-1-infected patients on antiretroviral therapy (plasma HIV-1 RNA levels ≤200 copies/mL; CD4+ T-cell counts ≥500 cells/mm3) were randomly allocated 5:1 to receive vaccine or placebo. At weeks 0, 4, and 12, 4 consecutive cohorts received 3000 μg HIV MAG pDNA with 0, 50, 250, or 1000 μg of IL-12 pDNA by IM-EP. A fifth cohort received HIV MAG pDNA and 1000 mg of IL-12 pDNA by standard IM injection. Results: CD4+ T cells expressing IL-2 in response to Gag and Pol and interferon-g responses to Gag, Pol, and Env increased from baseline to week 14 in the low-dose (50-μg) IL-12 arm vs. placebo (P < 0.05; intracellular cytokine staining). The total increase in the IL-2-expressing CD4+ T-cell responses to any antigen was also higher in the low-dose IL-12 arm vs. placebo (P = 0.04). Cytokine responses by CD8 T cells to HIV antigens were not increased in any vaccine arm relative to placebo. Conclusions: HIV-1 MAG/low-dose IL-12 DNA vaccine delivered by IM-EP augmented CD4+ but not CD8+ T-cell responses to multiple HIV-1 antigens. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15254135
- Volume :
- 71
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- JAIDS: Journal of Acquired Immune Deficiency Syndromes
- Publication Type :
- Academic Journal
- Accession number :
- 112610917
- Full Text :
- https://doi.org/10.1097/QAI.0000000000000830