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Total phenol analysis of weakly supported water using a laccase-based microband biosensor.
- Source :
-
Analytica Chimica Acta . Feb2016, Vol. 907, p45-53. 9p. - Publication Year :
- 2016
-
Abstract
- The monitoring of phenolic compounds in wastewaters in a simple manner is of great importance for environmental control. Here, a novel screen printed laccase-based microband array for in situ , total phenol estimation in wastewaters and for water quality monitoring without additional sample pre-treatment is presented. Numerical simulations using the finite element method were utilized for the characterization of micro-scale graphite electrodes. Anodization followed by covalent modification was used for the electrode functionalization with laccase. The functionalization efficiency and the electrochemical performance in direct and catechol-mediated oxygen reduction were studied at the microband laccase electrodes and compared with macro-scale electrode structures. The reduction of the dimensions of the enzyme biosensor, when used under optimized conditions, led to a significant improvement in its analytical characteristics. The elaborated microsensor showed fast responses towards catechol additions to tap water – a weakly supported medium – characterized by a linear range from 0.2 to 10 μM, a sensitivity of 1.35 ± 0.4 A M −1 cm −2 and a dynamic range up to 43 μM. This enhanced laccase-based microsensor was used for water quality monitoring and its performance for total phenol analysis of wastewater samples from different stages of the cleaning process was compared to a standard method. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHENOL
*WATER analysis
*BIOSENSORS
*PHENOLS
*LACCASE
Subjects
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 907
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 112628709
- Full Text :
- https://doi.org/10.1016/j.aca.2015.12.006