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Enhanced conjugation stability and blood circulation time of macromolecular gadolinium-DTPA contrast agent.
- Source :
-
Materials Science & Engineering: C . Apr2016, Vol. 61, p659-664. 6p. - Publication Year :
- 2016
-
Abstract
- In this study, we prepared macromolecular MR T1 contrast agent: pullulan-conjugated Gd diethylene triamine pentaacetate (Gd-DTPA-Pullulan) and estimated residual free Gd 3 + , chelation stability in competition with metal ions, plasma and tissue pharmacokinetics, and abdominal MR contrast on rats. Residual free Gd 3 + in Gd-DTPA-Pullulan was measured using colorimetric spectroscopy. The transmetalation of Gd 3 + incubated with Ca 2 + was performed by using a dialysis membrane (MWCO 100–500 Da) and investigated by ICP-OES. The plasma concentration profiles of Gd-DTPA-Pullulan were estimated after intravenous injection at a dose 0.1 mmol/kg of Gd. The coronal-plane abdominal images of normal rats were observed by MR imaging. The content of free Gd 3 + , the toxic residual form, was less than 0.01%. Chelation stability of Gd-DTPA-Pullulan was estimated, and only 0.2% and 0.00045% of Gd 3 + were released from Gd-DTPA-Pullulan after 2 h incubation with Ca 2 + and Fe 2 + , respectively. Gd-DTPA-Pullulan displayed the extended plasma half-life (t 1/2,α = 0.43 h, t 1/2,β = 2.32 h), much longer than 0.11 h and 0.79 h of Gd-EOB-DTPA. Abdominal MR imaging showed Gd-DTPA-Pullulan maintained initial MR contrast for 30 min. The extended plasma half-life of Gd-DTPA-Pullulan probably allows the prolonged MR acquisition time in clinic with enhanced MR contrast. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09284931
- Volume :
- 61
- Database :
- Academic Search Index
- Journal :
- Materials Science & Engineering: C
- Publication Type :
- Academic Journal
- Accession number :
- 112675098
- Full Text :
- https://doi.org/10.1016/j.msec.2016.01.008