Back to Search
Start Over
Development of a quantitative immuno-affinity test column assay for on-site screening of clindamycin residues in milk.
- Source :
-
International Dairy Journal . Apr2016, Vol. 55, p59-63. 5p. - Publication Year :
- 2016
-
Abstract
- A sensitive and specific monoclonal antibody against clindamycin (CLIN) was produced and used to develop an immuno-affinity test column (IATC) assay for on-site screening of CLIN residues in milk. The qualitative limit of detection of the IATC assay, estimated as 1.0 μg L −1 by visual detection, was sufficient to measure maximum residue levels for lincosamide antibiotics in milk. The quantitative IATC assay resulted in a lower detection limit (0.11 μg L −1 ) by evaluating the colour intensity of the test layers, which was approximately 9 times greater sensitivity than visual detection. During the spike and recovery test, the average recoveries ranged from 76% to 114% at different spiked levels, and the intra-/interday coefficients of variation were in the range 9.6–16.7%. The detection time was shortened to 20 min, whereas the sensitivity was comparable with a traditional ELISA. The IATC assay shows promise for on-site screening of CLIN residues in milk. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09586946
- Volume :
- 55
- Database :
- Academic Search Index
- Journal :
- International Dairy Journal
- Publication Type :
- Academic Journal
- Accession number :
- 112677366
- Full Text :
- https://doi.org/10.1016/j.idairyj.2015.12.002