雪松不同部位中原花青素的含量测定及其清除 DPPH 自由基的活性.

Cedrus deodara (Pinaceae family), which consists of C. deodara, C. libani, C. brevifolia and C. atlantica, is an evergreen tree growing extensively on the slopes of the Himalayas. Its leaves was a kind of chinese medicine for a long history. Its main chemical constituents include flavonoids, phenylpropanoids, organicacid, triterpenes, steroids, polysaccharides leaf glue and so on. The major pharmacological activities of pine needles of C. deodara include antitumor, antioxidation, improving memory, antibacterial and antivirus, ect. In order to determine proanthocyanidin content and DPPH radical scavenging activity of different parts from C. deodara. In this study, Fe3+ ion was used for catalysator, n-BuOH:HCl (95:5) was chromogenic agent and the detection wave-lengths were at 550 nm. And the content of proanthocyanidin was determined in different parts from C. deodara by ultraviolet-visible spectrophotometer. And antioxidant activity of extract from different parts of C. deodara were investigated by scavenging 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH). The results showed that proanthocyanidins content in different parts of C. deodara was pine needles (12.93%), inflorescence (12.93%),bark (12.00%), wood (6.41%), tender branchlets (3.37%). The extract from different parts of C. deodara had good antioxidant activity. In a certain concentration scope, there was a strong positive correlation between the scavenging activity and the concentrations. The order of IC50 was tender branchlets > pine needles > wood> bark > inflorescence. Except for branchlets, their DPPH scavenging activities were around 90% while the concentration of the extract from different parts was 0.8 mg·mL-1. The results indicated that there were higher proanthocyanidins content and antioxidant activities in different parts of C. deodara, which provide the reference on comprehensive development and utilization of C. deodara. [ABSTRACT FROM AUTHOR]