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Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance.

Authors :
Casado Muñoz, María del Carmen
Benomar, Nabil
Ennahar, Saïd
Horvatovich, Peter
Lavilla Lerma, Leyre
Knapp, Charles W.
Gálvez, Antonio
Abriouel, Hikmate
Source :
International Journal of Food Microbiology. Apr2016, Vol. 222, p8-15. 8p.
Publication Year :
2016

Abstract

Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required to discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic responses of Lactobacillus pentosu s MP-10, a potentially probiotic lactic acid bacteria isolated from brines of naturally fermented Aloreña green table olives, exposed to sub-lethal concentrations of antibiotics (amoxicillin, chloramphenicol and tetracycline) and biocides (benzalkonium chloride and triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as the up-regulation of protein synthesis, and the down-regulation of carbohydrate metabolism and energy production. The antimicrobials appeared to have altered Lb. pentosus MP-10 physiology to achieve a gain of cellular energy for survival. For example, biocide-adapted Lb. pentosu s MP-10 exhibited a down-regulated phosphocarrier protein HPr and an unexpressed oxidoreductase. However, protein synthesis was over-expressed in antibiotic- and biocide-adapted cells (ribosomal proteins and glutamyl-tRNA synthetase), possibly to compensate for damaged proteins targeted by antimicrobials. Furthermore, stress proteins, such as NADH peroxidase (Npx) and a small heat shock protein, were only over-expressed in antibiotic-adapted Lb. pentosu s MP-10. Results showed that adaptation to sub-lethal concentrations of antimicrobials could be a good way to achieve desirable robustness of the probiotic Lb. pentosu s MP-10 to various environmental and gastrointestinal conditions (e.g., acid and bile stresses). [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01681605
Volume :
222
Database :
Academic Search Index
Journal :
International Journal of Food Microbiology
Publication Type :
Academic Journal
Accession number :
113188179
Full Text :
https://doi.org/10.1016/j.ijfoodmicro.2016.01.012