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Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato.
- Source :
-
Genome . 2016, Vol. 59 Issue 3, p173-183. 11p. - Publication Year :
- 2016
-
Abstract
- Fusarium crown and root rot is a severe fungal disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In this study, the genomic location of the FORL-resistance locus was determined using a set of molecular markers on chromosome 9 and an F2 population derived from FORL-resistant inbred 'AV107-4' ( Solanum lycopersicum) × susceptible 'L3708' ( Solanum pimpinellifolium). Bioassay performed using Korean FORL strain KACC 40031 showed single dominant inheritance of FORL resistance in the F2 population. In all, 13 polymerase chain reaction-based markers encompassing approximately 3.6-72.0 Mb of chromosome 9 were developed based on the Tomato-EXPEN 2000 map and SolCAP Tomato single nucleotide polymorphism array analysis. These markers were genotyped on 345 F2 plants, and the FORL-resistance locus was found to be present on a pericentromeric region of suppressed chromosomal recombination in chromosome 9. The location of the FORL-resistance locus was further confirmed by testing these markers against diverse commercial tomato and stock cultivars resistant to FORL. A restriction fragment length polymorphism marker, PNU-D4, located at approximately 6.1 Mb of chromosome 9 showed the highest match with the resistance locus and was used for conducting high-resolution melting analysis for marker-assisted selection of FORL resistance. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08312796
- Volume :
- 59
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Genome
- Publication Type :
- Academic Journal
- Accession number :
- 113416838
- Full Text :
- https://doi.org/10.1139/gen-2015-0115