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Recognition of MCF-7 human breast carcinoma cells using silica-encapsulated fluorescent nanoparticles modified with aminophenylboronic acid.
- Source :
-
Microchimica Acta . Mar2016, Vol. 183 Issue 3, p1115-1122. 8p. - Publication Year :
- 2016
-
Abstract
- A method is reported for recognizing MCF-7 human breast carcinoma cells based on silica-encapsulated nanoparticles modified with aminophenylboronic acid which can recognize sialic acid on cell surfaces. Gold@rhodamine B nanoparticles were coated with aminophenylboronic acid and used to capture MCF-7 cells. It is found that the presence of gold NPs was favorable to prepare nanoparticles easily and that they were extraordinarily biocompatible with MCF-7 cells. The experimental results confirmed that the nanoparticles can be used to target breast carcinoma cells using HS 578Bst normal breast cells as the negative control. The MCF-7 cells were imaged by laser scanning microscopy and showed strong red fluorescence in dark field. An MTT test revealed an 82 % viability of cells when 50 mg · mL fluorescent probe was used in the incubation experiments. The results exhibited that the NPs are innocuous and stable. In our perception, the method has a larege potential for early diagnosis of breast cancer due to high affinity between nanoparticles and the breast carcinoma cells. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 183
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 113485587
- Full Text :
- https://doi.org/10.1007/s00604-015-1736-9