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Resistance to Fas-mediated apoptosis: activation of Caspase 3 is regulated by cell cycle regulator p21WAF1 and IAP gene family ILP.

Authors :
Suzuki, Atsushi
Tsutomi, Yumi
Akahane, Kouichi
Araki, Takashi
Miura, Masayuki
Source :
Oncogene. 8/27/98, Vol. 17 Issue 8, p931-939. 9p. 1 Color Photograph, 4 Diagrams, 5 Graphs.
Publication Year :
1998

Abstract

The death receptor Fas transduces apoptotic death signaling mediated by caspases. In the present study, human hepatoma HepG2 cells showed the Fas-mediated apoptosis mediated by caspase, especially caspase 3, only in the presence of actinomycin D. Interestingly, cytosolic proteins extracted from intact HepG2 cells induced caspase 3 inactivation. Our results reveal that this inactivation was triggered by the direct inhibition of activated caspase 3 by IAP gene family ILP. In addition, a 53 kDa protein was co-immunoprecipitated with anti-human caspase 3 antibody from intact HepG2 cells. This protein was a complex-protein of procaspase 3 and the cell cycle regulator p21WAF1 (p21). P21 bound to only procaspase 3, but not to activated caspase 3. We also demonstrate that p21 protein-loaded HepG2 cells resist to Fas-mediated apoptosis even in the presence of actinomycin D. Here we report that caspase 3 inactivation for the resistance to Fas-mediated apoptosis is induced by a procaspase 3/p21 complex formation and direct inhibition of activated caspase 3 by ILP. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09509232
Volume :
17
Issue :
8
Database :
Academic Search Index
Journal :
Oncogene
Publication Type :
Academic Journal
Accession number :
11352151
Full Text :
https://doi.org/10.1038/sj.onc.1202021