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CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells.
- Source :
-
BioMed Research International . 11/30/2015, Vol. 2015, p1-7. 7p. - Publication Year :
- 2015
-
Abstract
- MicroRNA 155 (miR-155) is a key proinflammatory regulator in clinical and experimental rheumatoid arthritis (RA). Here we generated a miR-155 genome knockout (GKO) RAW264.7 macrophage cell line using the clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CAS9) technology. While upregulating the Src homology-2 domain-containing inositol 5-phosphatase 1 (SHIP1), the miR-155 GKO line is severely impaired in producing proinflammatory cytokines but slightly increased in osteoclastogenesis upon treatment with receptor activator of nuclear factor-κB ligand (RANKL). Taken together, our results suggest that genome editing of miR-155 holds the potential as a therapeutic strategy in RA. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENETICS of rheumatoid arthritis
*RHEUMATOID arthritis treatment
*ANIMAL experimentation
*BIOLOGICAL models
*CELL culture
*CYTOKINES
*ENZYME-linked immunosorbent assay
*GENETIC techniques
*GENOMES
*INFLAMMATORY mediators
*INTERLEUKINS
*LIGANDS (Biochemistry)
*MACROPHAGES
*MICE
*GENETIC mutation
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*RNA
*T-test (Statistics)
*TUMOR necrosis factors
*WESTERN immunoblotting
*DNA-binding proteins
*LIPOPOLYSACCHARIDES
*DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2015
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 113630136
- Full Text :
- https://doi.org/10.1155/2015/326042