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The effect of Toll-like receptor 4 on β2-glycoprotein I-induced B cell activation in mouse model.
- Source :
-
Molecular Immunology . Mar2016, Vol. 71, p78-86. 9p. - Publication Year :
- 2016
-
Abstract
- Our previous study demonstrated that Toll-like receptor 4 (TLR4) plays a vital role in the maturation of bone marrow-derived dendritic cells (BMDCs) from the mice immunized with human β 2 -glycoprotein I (β 2 GPI). However, the roles of TLR4 in the activation of B cells and production of anti-β 2 GPI antibodies in vivo have been rarely studied. This study aimed to investigate the activation of B cells from TLR4-defective (C3H/HeJ) and TLR4-intact (C3H/HeN) mice pre-immunized with human β 2 GPI. After β 2 GPI injection, the level of anti-β 2 GPI antibody in the serum of TLR4-defective and TLR4-intact mice was gradually increased and the number and size of germinal centers in the spleen were also significantly increased. Compared with C3H/HeJ mice, we observed significantly higher anti-β 2 GPI antibody titer and more germinal centers in C3H/HeN mice. Moreover, the β 2 GPI-induced expression of CD40L, CD40, CD80, CD86 and MHC II in C3H/HeN mice was significantly higher than that in C3H/HeJ mice. Furthermore, the β 2 GPI-induced expression of B cell activating factor (BAFF) in the spleen and IL-6 and IL-10 in B cells from C3H/HeN mice was also significantly increased compared to C3H/HeJ mice. Taken together, our results suggest that TLR4 is required for the activation of B cells and the production of autoantibody in mice treated with β 2 GPI, but the immunological mechanisms of antiphospholipid syndrome (APS) need further investigation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 71
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 113729262
- Full Text :
- https://doi.org/10.1016/j.molimm.2016.01.012