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Effects of Progesterone and 18-Methyl Levonorgestrel on Osteoblastic Cells.
- Source :
-
Endocrine Research . Nov2003, Vol. 29 Issue 4, p483-501. 19p. - Publication Year :
- 2003
-
Abstract
- OBJECTIVE: To study the effects of progesterone (P) and 18-methyl levonorgestrel (LNG) on the expression of P receptors (PRA and PRB), c-fos, c-jun, and osteocalcin in the human osteosarcoma cell line MG-63 and human normal osteoblasts in order to understand the mechanism of progestin action on the proliferation and differentiation of osteoblasts. METHODS: Cell proliferation was tested by MTT assay. The expression of PR, c-fos, c-jun, and osteocalcin was measured by semi-quantitative RT-PCR, Western immunoblot or immunocytochemistry. RESULTS: Progesterone and LNG (10−10–10−6 M) stimulated cell proliferation in a dose-dependent manner. Progesterone and LNG did not affect the expression of PRA and PRB mRNA and protein, but c-fos and c-jun mRNA and protein were upregulated in a dose-dependent manner. The expression of osteocalcin mRNA was also increased in human osteoblasts in a dose and time-dependent manner with greater effects of LNG than P, while the expression of osteocalcin mRNA in MG-63 cells was not changed by P or LNG. CONCLUSION: Progesterone and LNG promote osteocalcin gene transcription by stimulating the expression of c-fos and c-jun, and result in osteoblast proliferation and differentiation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROGESTERONE
*CELL proliferation
*IMMUNOBLOTTING
*MESSENGER RNA
*OSTEOSARCOMA
Subjects
Details
- Language :
- English
- ISSN :
- 07435800
- Volume :
- 29
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Endocrine Research
- Publication Type :
- Academic Journal
- Accession number :
- 11398214
- Full Text :
- https://doi.org/10.1081/ERC-120026954