菊花转录因子ＣｍＭＹＢ５９ 的克隆与表达特性分析.

[Objectives]We had a preliminary study on the function of CmMYB59 and explained its response to the cold stress. [Methods]In this study,a novel MYB and its alternative splicing transcript were isolated from Chrysanthemum morifolium‘Jinba’by RT-PCR and RACE methods. The expression of CmMYB59 in different tissues and cold treatment conditions was studied by qRT-PCR. Furthermore,its transcriptional activation and subcellular localization were analyzed by the yeast one-hybrid assay and transient expression in onion epidermal cells respectively. [Results]The full length of this gene was 904 bp,containing an open reading frame of 768 bp,encoding 255 amino acids,with relative molecular mass of 61.99×103. The multiple alignment of the protein sequences showed the protein had a typical R2R3-MYB domain and was most closed to AtMYB59. Thus,the gene was named CmMYB59. qRT-PCR showed that the expression of CmMYB59 was the highest in roots,higher in stems and leaves,the lowest in shoot tip and floral organ,and it was responsive to the cold stress. The subcellular localization indicated that the protein was located in nucleus. The yeast one-hybrid assay showed that the CmMYB59 protein had a transcriptional activation function. [Conclusions]It is speculated that CmMYB59 might be related to cell division,participating in the development process of root,and being associated with cold stress. [ABSTRACT FROM AUTHOR]