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Th17 expression and IL-17 levels in laryngeal squamous cell carcinoma patients.
- Source :
-
Acta Oto-Laryngologica . May2016, Vol. 136 Issue 5, p484-490. 7p. - Publication Year :
- 2016
-
Abstract
- ConclusionThe Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging.ObjectivesTo investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma.MethodEighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively.ResultsTh17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p < 0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p < 0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p < 0.01 for mRNA levels,p < 0.05 for protein levels). [ABSTRACT FROM PUBLISHER]
- Subjects :
- *RNA analysis
*ACADEMIC medical centers
*CHI-squared test
*CONFIDENCE intervals
*ENZYME-linked immunosorbent assay
*FISHER exact test
*FLOW cytometry
*IMMUNOHISTOCHEMISTRY
*INTERLEUKINS
*RESEARCH methodology
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*SQUAMOUS cell carcinoma
*STATISTICS
*T cells
*T-test (Statistics)
*TISSUE culture
*TUMOR classification
*DATA analysis
*REVERSE transcriptase polymerase chain reaction
*DATA analysis software
*DESCRIPTIVE statistics
*MANN Whitney U Test
LARYNGEAL tumors
Subjects
Details
- Language :
- English
- ISSN :
- 00016489
- Volume :
- 136
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Acta Oto-Laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 114327429
- Full Text :
- https://doi.org/10.3109/00016489.2015.1126857