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Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons.
- Source :
-
Angewandte Chemie International Edition . 4/11/2016, Vol. 55 Issue 16, p4933-4937. 5p. - Publication Year :
- 2016
-
Abstract
- Newly synthesized proteins constitute an important subset of the proteome involved in every cellular process, yet existing chemical tools used to study them have major shortcomings. Herein we report a suite of cell-permeable puromycin analogues capable of being metabolically incorporated into newly synthesized proteins in different mammalian cells, including neuronal cells. Subsequent labeling with suitable bioorthogonal reporters, in both fixed and live cells, enabled direct imaging and enrichment of these proteins. By taking advantage of the mutually orthogonal reactivity of these analogues, we showed multiplexed labeling of different protein populations, as well as quantitative measurements of protein dynamics by fluorescence correlation spectroscopy, could be achieved in live-cell environments. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PUROMYCIN
*PROTEINS
*CHEMICAL synthesis
*FLUORESCENCE spectroscopy
*NEURONS
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 55
- Issue :
- 16
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 114538969
- Full Text :
- https://doi.org/10.1002/anie.201511030