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Hypermethylation of DLX4 predicts poor clinical outcome in patients with myelodysplastic syndrome.
- Source :
-
Clinical Chemistry & Laboratory Medicine . May2016, Vol. 54 Issue 5, p865-871. 7p. - Publication Year :
- 2016
-
Abstract
- Background: Hypermethylation of DLX4 (distal-less homeobox 4) has been disclosed in a variety of cancers. Our work was aimed to examine the pattern of DLX4 methylation and further investigate its clinical relevance in patients with myelodysplastic syndrome (MDS). Methods: Real-time quantitative methylation-specific PCR and bisulfite sequencing PCR were carried out to detect the level of DLX4 methylation. Clinical significance of DLX4 methylation was analyzed between the DLX4 hypermethylated and non-hypermethylated patients. Results: DLX4 was significantly hypermethylated in MDS patients than controls (p<0.001). No significant differences were observed between the hypermethylated and non-hypermethylated MDS patients in white blood cells, platelets, age, WHO classifications, FAB classifications, IPSS risks, and common gene mutations (p>0.05). However, DLX4 hypermethylated patients tended to have higher hemoglobin (HB) than DLX4 non-hypermethylated patients (p=0.079). Moreover, there was a trend that male patients, poor karyotype patients, and IPSS Int-2/High patients had a higher frequency of DLX4 hypermethylation (p=0.067, 0.065, and 0.068). DLX4 hypermethylated patients had significantly shorter overall survival than DLX4 non-hypermethylated patients (p=0.004). Multivariate analysis confirmed the prognostic value of DLX4 methylation in MDS patients (p<0.001). Conclusions: Our study indicated that DLX4 hypermethylation was a frequent event and acted as an independent prognostic biomarker in de novo MDS patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14346621
- Volume :
- 54
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Clinical Chemistry & Laboratory Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 114787600
- Full Text :
- https://doi.org/10.1515/cclm-2015-0536