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Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines.

Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines.

Authors :
Riku Shirayama
Masaki Shoji
Nongluk Sriwilaijaroen
Hiroaki Hiramatsu
Yasuo Suzuki
Takashi Kuzuhara
Source :
Drug Discoveries & Therapeutics. Apr2016, Vol. 10 Issue 2, p109-113. 5p.
Publication Year :
2016

Abstract

To find a novel influenza inhibitor targeting the endonuclease activity of influenza A virus polymerase acidic protein (PA), which is essential for the acquisition of primers for viral mRNA transcription, seven Kampo extracts were tested in vitro for their ability to inhibit endonuclease activity of the recombinant PA protein that was expressed and purified from Escherichia coli. The Kampo medicines Kakkonto, Shosaikoto, Saikokeishito, Keishito, Maobushisaishinto, and Maoto, but not Chikujountanto, inhibited PA endonuclease activity in a dose-dependent manner. Our results indicate that Kampo medicines are good sources providing a structural lead for optimization of an influenza endonuclease inhibitor. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
18817831
Volume :
10
Issue :
2
Database :
Academic Search Index
Journal :
Drug Discoveries & Therapeutics
Publication Type :
Academic Journal
Accession number :
115256414
Full Text :
https://doi.org/10.5582/ddt.2016.01010