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Chiral copper(II) complex based on natural product rosin derivative as promising antitumour agent.
- Source :
-
Journal of Photochemistry & Photobiology B: Biology . Jul2016, Vol. 160, p43-52. 10p. - Publication Year :
- 2016
-
Abstract
- To evaluate the biological preference of chiral drug candidates for molecular target DNA, the synthesis and characterization of a chiral copper(II) complex ( 2 ) of a chiral ligand N , N ′-(pyridin-2-ylmethylene) dehydroabietylamine ( 1 ) was carried out. The interactions of 1 and 2 with salmon sperm DNA were investigated by viscosity measurements, UV, fluorescence and circular dichroism (CD) spectroscopic techniques. Absorption spectral, emission spectral and viscosity analysis reveal that 1 and 2 interacted with DNA through intercalation and 2 exhibited a higher DNA binding ability. In the absence/presence of ascorbic acid, 1 and 2 cleaved supercoiled pBR322 DNA by single-strand and 2 displayed stronger DNA cleavage ability. In addition, in vitro cytotoxicity of 1 and 2 against HeLa, SiHa, HepG-2 and A431 cancer cell lines study show that they exhibited effective cytotoxicity against the tested cell lines, notably, 2 showed a superior cytotoxicity than the widely used drug cisplatin under identical conditions, indicating it has the potential to act as effective anticancer drug. Flow cytometry analysis indicates 2 produced death of HeLa cancer cells through an apoptotic pathway. Cell cycle analysis demonstrates that 2 mainly arrested HeLa cells at the S phase. The study represents the first step towards understanding the mode of the promising chiral rosin-derivative based copper complexes as chemotherapeutics. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10111344
- Volume :
- 160
- Database :
- Academic Search Index
- Journal :
- Journal of Photochemistry & Photobiology B: Biology
- Publication Type :
- Academic Journal
- Accession number :
- 115798647
- Full Text :
- https://doi.org/10.1016/j.jphotobiol.2016.03.044