Cissus quadrangularis prevented the ovariectomy induced oxidative stress in the femur of adult albino rats.

The increasing evidence suggesting the role of free radicals in bone resorption and bone loss prompted us to explore whether the consumption of antioxidant rich medicinal plant C. quadrangularis modifies antioxidant status in ovariectomized rats. Methods Twenty four female adult rats, 90 days old showing regular estrous cycles were used for the present study. The animals were divided into two groups. The Group-1 rats (n = 6) were sham operated and Group-II rats were bilaterally ovariectomized (n = 18) and treated with C. quadrangularis for sixty days (100 mg/ kg body weight and 250 mg/kg body weight). After sixty days, the rats were killed, femora were dissected out, minced and homogenized in Tris-HCl buffer (pH 7.4) and the supernatant was collected and used for biochemical assays. Results Ovariectomy registered a decrease (p<0.05) in the activities of SOD, GPx, GST, ALP, collagen content and increased (p<0.05) the activities of TRAP and lipid peroxidation. Simultaneous administration of C. quadrangularis maintained the enzyme activities in ovariectomized rats. Conclusion C. quadrangularis , a natural herb may be used to treat the estrogen deficiency/menopause onset and ovariectomy induced oxidative stress. [ABSTRACT FROM AUTHOR]