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Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1 / Khdc3l Gene: A Comparison with Ovine Species.

Authors :
Zahmatkesh, Azadeh
Ansari Mahyari, Saeid
Daliri Joupari, Morteza
Rahmani, Hamidreza
Shirazi, Abolfazl
Amiri Roudbar, Mahmood
Ansari Majd, Saeid
Source :
Animal Biotechnology. 2016, Vol. 27 Issue 3, p174-181. 8p.
Publication Year :
2016

Abstract

Maternal effect genes have highly impressive effects on pre-implantation development.Filia/Ecat1/Khdc3lis a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigatedFiliamRNA expression in bovine oviduct, 30- to 40-day fetus, liver, heart, lung, and oocytes (as a positive control), by RT-PCR and detected it only in oocytes. A 443 bp fragment was amplified only in oocytes and was sequenced as a part of bovine predictedFiliamRNA. We analyzed bovine and ovine Filia N-terminal peptide sequence in PHYRE2, and a KH domain was predicted. Protein alignment using ClustalW indicated a highly identical N-terminal extention between the 2 species. Immunohistochemical analysis using anti-bovine Filia antibody showed the expression of Filia protein in the zone surrounding the nuclear membrane, and in the subcortex of ovine oocytes of primary and antral follicles. However, in the bovine, Filia has been found through the oocyte cytoplasm of antral follicles, and here it is further confirmed in the primary follicles. Our data suggests a difference in Filia expression pattern between cow and sheep, although the sequence is highly conserved. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10495398
Volume :
27
Issue :
3
Database :
Academic Search Index
Journal :
Animal Biotechnology
Publication Type :
Academic Journal
Accession number :
115862630
Full Text :
https://doi.org/10.1080/10495398.2016.1157081