癌基因 Src 在骨肉瘤细胞侵袭伪足形成中的作用.

Objective: To investigate the effects of oncogene Src on invadopodia formation of osteosarcoma cells(HT-1080) in vitro. Methods: Src sh RNA lentiviral vector was constructed, packaged in HEK293 T cells. And HT-1080 cells were infected, then screened by puromycin. And then stablely Src gene silencing osteosarcoma cell line HT-1080-sh Src was obtained. Quantification PCR and Western Blot were carried out to detect the gene silencing efficiency. Invadopodia formation was observed by in situ gelatin zymography. In vitro invasion assays were used to detect the effects of downregulation Src expression on invasive ability of HT-1080 cells. Results: Stablely Src gene silencing osteosarcoma cell line HT-1080-sh Src and control cell line HT-1080-shluc were successfully constructed. Quantification PCR and Western Blot showed, compared with HT-1080-shluc cells, the expression of Src in HT-1080-sh Src cells decreased more than 3 times. Invadopodia formation, the ability to degradate the extracellular matrix and the invasive ability were significantly inhibited when Src expression was downregulated in HT-1080 cells. Conclusion: Oncogene Src is involved in regulating invadopodia formation in osteosarcoma cells HT-1080, and promoting tumor invasion and metastasis. [ABSTRACT FROM AUTHOR]