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Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes.
- Source :
-
Tetrahedron . Jul2016, Vol. 72 Issue 27/28, p4085-4090. 6p. - Publication Year :
- 2016
-
Abstract
- A new fluorogenic substrate Ub-ACC (ubiquitin C-terminal 7-amino-4-carbamoylmethyl-coumarin) was developed for DUB (deubiquitinating enzyme) activity assays. This substrate can be synthesized with higher efficiency than the classical DUB substrate, Ub-AMC (ubiquitin C-terminal 7-amino-4-methylcoumarin). DUB assays using UCH-L3, OTUD2 and USP30 demonstrated that Ub-ACC shows nearly 2-fold higher sensitivity than Ub-AMC. [ABSTRACT FROM AUTHOR]
- Subjects :
- *UBIQUITIN
*COUMARINS
*PROTEOLYTIC enzymes
*PROTEIN folding
*PROTEOLYSIS
Subjects
Details
- Language :
- English
- ISSN :
- 00404020
- Volume :
- 72
- Issue :
- 27/28
- Database :
- Academic Search Index
- Journal :
- Tetrahedron
- Publication Type :
- Academic Journal
- Accession number :
- 115977234
- Full Text :
- https://doi.org/10.1016/j.tet.2016.05.041