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Crystal structure of the TK2203 protein from Thermococcus kodakarensis, a putative extradiol dioxygenase.
- Source :
-
Acta Crystallographica: Section F, Structural Biology Communications . Jun2016, Vol. 72 Issue 6, p427-433. 6p. - Publication Year :
- 2016
-
Abstract
- The TK2203 protein from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (262 residues, 29 kDa) is a putative extradiol dioxygenase catalyzing the cleavage of C-C bonds in catechol derivatives. It contains three metal-binding residues, but has no significant sequence similarity to proteins for which structures have been determined. Here, the first crystal structure of the TK2203 protein was determined at 1.41 Å resolution to investigate its functional role. Structure analysis reveals that this protein shares the same fold and catalytic residues as other extradiol dioxygenases, strongly suggesting the same enzymatic activity. Furthermore, the important region contributing to substrate selectivity is discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BACTERIAL protein structure
*THERMOCOCCUS kodakaraensis
*SCISSION (Chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 2053230X
- Volume :
- 72
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F, Structural Biology Communications
- Publication Type :
- Academic Journal
- Accession number :
- 116146730
- Full Text :
- https://doi.org/10.1107/S2053230X16006920