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Molecular Imaging for Comparison of Different Growth Factors on Bone Marrow-Derived Mesenchymal Stromal Cells’ Survival and Proliferation In Vivo.
- Source :
-
BioMed Research International . 6/21/2016, Vol. 2016, p1-10. 10p. - Publication Year :
- 2016
-
Abstract
- Introduction. Bone marrow-derived mesenchymal stromal cells (BMSCs) have emerged as promising cell candidates but with poor survival after transplantation. This study was designed to investigate the efficacy of VEGF, bFGF, and IGF-1 on BMSCs’ viability and proliferation both in vivo and in vitro using bioluminescence imaging (BLI). Methods. BMSCs were isolated from β-actin-Fluc+ transgenic FVB mice, which constitutively express firefly luciferase. Apoptosis was induced by hypoxia preconditioning for up to 24 h followed by flow cytometry and TUNEL assay. 106 BMSCs with/without growth factors were injected subcutaneously into wild type FVB mice’s backs. Survival of BMSCs was longitudinally monitored using bioluminescence imaging (BLI) for 5 weeks. Protein expression of Akt, p-Akt, PARP, and caspase-3 was detected by Western blot. Results. Hypoxia-induced apoptosis was significantly attenuated by bFGF and IGF-1 compared with VEGF and control group in vitro (P<0.05). When combined with matrigel, IGF-1 showed the most beneficial effects in protecting BMSCs from apoptosis in vivo. The phosphorylation of Akt had a higher ratio in the cells from IGF-1 group. Conclusion. IGF-1 could protect BMSCs from hypoxia-induced apoptosis through activation of p-Akt/Akt pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANALYSIS of variance
*ANALYTICAL biochemistry
*ANIMAL experimentation
*HYPOXEMIA
*APOPTOSIS
*BIOLOGICAL assay
*COLLECTION & preservation of biological specimens
*CELL physiology
*COMPARATIVE studies
*DIAGNOSTIC imaging
*FLOW cytometry
*GENE expression
*GROWTH factors
*LUMINESCENCE spectroscopy
*MICE
*MOLECULAR diagnosis
*PROBABILITY theory
*PROTEINS
*RESEARCH funding
*SOMATOMEDIN
*STEM cells
*TRANSGENIC animals
*WESTERN immunoblotting
*DATA analysis software
*DESCRIPTIVE statistics
*IN vitro studies
*IN vivo studies
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2016
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 116283690
- Full Text :
- https://doi.org/10.1155/2016/1363902