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Nipah Virus Matrix Protein Influences Fusogenicity and Is Essential for Particle Infectivity and Stability.
- Source :
-
Journal of Virology . Mar2016, Vol. 90 Issue 5, p2514-2522. 9p. - Publication Year :
- 2016
-
Abstract
- Nipah virus (NiV) causes fatal encephalitic infections in humans. To characterize the role of the matrix (M) protein in the viral life cycle, we generated a reverse genetics system based on NiV strain Malaysia. Using an enhanced green fluorescent protein (eGFP)-expressingMprotein-deleted NiV, we observed a slightly increased cell-cell fusion, slow replication kinetics, and significantly reduced peak titers compared to the parental virus. While increased amounts of viral proteins were found in the supernatant of cells infected with M-deleted NiV, the infectivity-to-particle ratio was more than 100-fold reduced, and the particles were less thermostable and of more irregular morphology. Taken together, our data demonstrate that theMprotein is not absolutely required for the production of cell-free NiV but is necessary for proper assembly and release of stable infectious NiV particles. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0022538X
- Volume :
- 90
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 116392268
- Full Text :
- https://doi.org/10.1128/JVI.02920-15