Back to Search
Start Over
Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms.
- Source :
-
Italian Journal of Food Safety . 2016, Vol. 5 Issue 2, p91-94. 4p. - Publication Year :
- 2016
-
Abstract
- This study was aimed at collecting data on presence, dissemination and persistence of Pseudomonas in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of Pseudomonadaceae was performed, 3-5 colonies/samples were selected for identification via 16SrDNA/opr/ polymerase chain reaction (PCR), and typed by enterobacterial-repetitive-intergenic-consensus (ERIC)-PCR. Pseudomonadaceae were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were Pseudomonas fluorescens (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, Pseudomonas spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by Pseudomonas spp. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PSEUDOMONADACEAE
*DAIRY farms
Subjects
Details
- Language :
- English
- ISSN :
- 22397132
- Volume :
- 5
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Italian Journal of Food Safety
- Publication Type :
- Academic Journal
- Accession number :
- 116685270
- Full Text :
- https://doi.org/10.4081/ijfs.2016.5652