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Identification of differentially expressed genes in salivary adenoid cystic carcinoma cells associated with metastasis.

Authors :
Wei Chen
Bing-Yao Liu
Xiang Zhang
Xiao-Ge Zhao
Gang Cao
Zhen Dong
Sen-Lin Zhang
Chen, Wei
Liu, Bing-Yao
Zhang, Xiang
Zhao, Xiao-Ge
Cao, Gang
Dong, Zhen
Zhang, Sen-Lin
Source :
Archives of Medical Science. Aug2016, Vol. 12 Issue 4, p881-888. 8p.
Publication Year :
2016

Abstract

<bold>Introduction: </bold>Salivary adenoid cystic carcinoma (SACC) is a frequent type of salivary gland cancer which is characterized by slow growth but high incidence of distant metastasis. We aimed to identify therapeutic targets which are associated with metastasis of SACC.<bold>Material and Methods: </bold>Total RNA was isolated from a low metastatic SACC cell line (ACC-2) and a highly metastatic SACC cell line (ACC-M), which was screened from ACC-2 by combination of in vivo selection and cloning in vitro. Then the total RNA was subjected to microarray analysis. Differentially expressed genes (DEGs) were screened from ACC-M compared with ACC-2, followed by Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Function annotation for DEGs also was performed. A protein-protein interaction network (PPI) was constructed for DEGs.<bold>Results: </bold>A total of 1128 DEGs were identified from ACC-M cells compared with ACC-2 cells. Both up- and down-regulated DEGs were enriched in different functions in biological process (BP), cellular component (CC) and molecular function (MF). Additionally, down-regulated DEGs were mainly enriched in "Apoptosis" and "Cytokine-cytokine receptor interaction" pathways which involved IFN-α1, NTRK1 and TGF-β1. In the PPI network, PIK3CA, PTPN11 and PIK3R1 had a number of nodes greater than 10.<bold>Conclusions: </bold>Transforming growth factor β1 might play a pivotal role during lung metastasis of SACC and be selected as a candidate target for treatment of metastatic SACC. IFNA1, NTRK1 and PIK3CA were also associated with tumor metastasis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17341922
Volume :
12
Issue :
4
Database :
Academic Search Index
Journal :
Archives of Medical Science
Publication Type :
Academic Journal
Accession number :
116702057
Full Text :
https://doi.org/10.5114/aoms.2016.60973