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Influenza Virus Induces Inflammatory Response in Mouse Primary Cortical Neurons with Limited Viral Replication.
- Source :
-
BioMed Research International . 7/21/2016, Vol. 2016, p1-7. 7p. - Publication Year :
- 2016
-
Abstract
- Unlike stereotypical neurotropic viruses, influenza A viruses have been detected in the brain tissues of human and animal models. To investigate the interaction between neurons and influenza A viruses, mouse cortical neurons were isolated, infected with human H1N1 influenza virus, and then examined for the production of various inflammatory molecules involved in immune response. We found that replication of the influenza virus in neurons was limited, although early viral transcription was not affected. Virus-induced neuron viability decreased at 6 h postinfection (p.i.) but increased at 24 h p.i. depending upon the viral strain. Virus-induced apoptosis and cytopathy in primary cortical neurons were not apparent at 24 h p.i. The mRNA levels of inflammatory cytokines, chemokines, and type I interferons were upregulated at 6 h and 24 h p.i. These results indicate that the influenza virus induces inflammatory response in mouse primary cortical neurons with limited viral replication. The cytokines released in viral infection-induced neuroinflammation might play critical roles in influenza encephalopathy, rather than in viral replication-induced cytopathy. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANIMAL experimentation
*APOPTOSIS
*BIOLOGICAL assay
*CELL culture
*CELL physiology
*CEREBRAL cortex
*CYTOKINES
*ENZYME-linked immunosorbent assay
*FLUORESCENT antibody technique
*HEMAGGLUTINATION tests
*INFLAMMATION
*INFLUENZA
*MICE
*MICROSCOPY
*NEURONS
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*T-test (Statistics)
*INFLUENZA A virus, H1N1 subtype
*DATA analysis software
*DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2016
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 116933474
- Full Text :
- https://doi.org/10.1155/2016/8076989