结直肠癌组织中 Id-1、MMP-9 的表达变化及其意义.

Objective To observe the expression changes of inhibitor of DNA binding-1 (Id-1 ) and matrix metallo-proteinase-9 (MMP-9) in the human colorectal carcinoma tissues and to discuss the clinical significance. Methods The expression of Id-1 and MMP-9 in 50 specimens of colorectal carcinoma tissues (observation group) and 50 specimens of normal colorectal tissues (control group) was detected by immunohistochemistry, RT-PCR and Western blotting. The cor-relations between their positive expression and the clinicopathological features were analyzed. Results The positive rates of Id-1 in the observation group and the control group were 72% and 24%, the positive rates of MMP-9 were 78% and 22%, respectively, and there were significant differences between these two groups (all P<0.05). The positive expression of Id-1 and MMP-9 was related with the depth of tumor invasion, TNM stage, lymph node metastasis, vessel invasion and liver metastasis (all P<0.05). The relative expression of Id-1 mRNA in the observation group and the control group was 0.96 ± 0. 03 and 0.20 ± 0.04, the relative expression of MMP-9 mRNA was 0.88 ± 0.04 and 0.21 ± 0.03, respectively, and there were significant differences (all P<0. 05). The relative expression of Id-1 protein was 0.82 ± 0.04 and 0.31 ± 0.02, the relative expression of MMP-9 in the observation group and the control group was 0.86 ± 0.05 and 0.29 ± 0.03, respectively, and there were significant differences between these two groups (all P<0.05). Conclusion The expression of Id-1 and MMP-9 was increased in the human colorectal carcinoma tissues, and the expression was related with tumor in-vasion, TNM stage, lymph node metastasis, liver metastasis and vessel invasion. [ABSTRACT FROM AUTHOR]