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Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation.
- Source :
-
BioResources . 2016, Vol. 11 Issue 3, p6393-6406. 14p. 2 Color Photographs, 1 Chart, 5 Graphs. - Publication Year :
- 2016
-
Abstract
- A robust process of purification, characterization, and application of endoglucanase from the agro-industrial waste was performed using solid state fermentation (SSF). Trichoderma harzianum as a micro-organism and wheat straw as a growth supportive substrate were used in SSF under pre-optimized conditions. The maximum activity of 480 ± 4.22 U/mL of endoglucanase was attained when a fermentation medium was inoculated using 10% inoculum size and 3% substrate concentration with pH = 5.5 at 35 °C for an optimized fermentation period. In comparison with crude extract, enzyme was 1.83-fold purified with a specific activity of 101.05 U/mg using Sephadex-G-100 column chromatography. Sodium dodecyl sulfate (SDS) poly-acrylamide gel electrophoresis revealed that the enzyme exhibited a low molecular weight of 43 kDa. The purified enzyme displayed maximum activity at pH = 6 and a temperature of 50 °C, respectively. The maximum activity (Vmax) of 156 U/mL and KM value of 63 µM were observed. Ethylenediaminetetraacetic acid (EDTA), SDS, and Hg2+ inhibited enzyme activity, while Co2+ and Mn2+ enhanced enzyme activity at 1 mM concentration. The maximum substrate affinity and specific activity of biosynthesized endoglucanase revealed that it can be potentially useful for industrial applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19302126
- Volume :
- 11
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- BioResources
- Publication Type :
- Academic Journal
- Accession number :
- 117104263
- Full Text :
- https://doi.org/10.15376/biores.11.3.6393-6406