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Orexin-A stimulates the expression of GLUT4 in a glucose dependent manner in the liver of orange-spotted grouper (Epinephelus coioides).

Authors :
Zhang, Cong
Sun, Caiyun
Wang, Bin
Yan, Peipei
Wu, Amin
Yang, Guokun
Li, Wensheng
Source :
Comparative Biochemistry & Physiology Part A: Molecular & Integrative Physiology. Sep2016, Vol. 199, p95-104. 10p.
Publication Year :
2016

Abstract

Orexins are hypothalamic neuropeptides involved in the central regulation of feeding behavior, sleep-wake cycle and other physiological functions. Orexin-A can regulate energy metabolism and increase glucose uptake, suggesting a role in glucose metabolism. In this study, we investigated the effects of orexin-A on GLUT4 mRNA and protein levels and the intracellular signaling mechanisms mediating orexin-A activity in the hepatocytes of grouper. Our results demonstrate that intraperitoneal injection of orexin-A increased the expression of GLUT4 in the liver, and this effect was significantly enhanced by co-injection of glucose. Treatment of primary cultured hepatocytes with either orexin-A or glucose alone had no effect on the expression of GLUT4, while co-treatment with orexin-A and glucose significantly increased the expression of GLUT4. This stimulatory effect was partially blocked by inhibitors to ERK1/2, JNK or p38 MAPK and was further blocked by an orexin receptor antagonist, which indicates that orexin-A could stimulate the expression of GLUT4 in a glucose dependent manner in primary hepatocytes via ERK1/2, JNK and p38 signaling. Our results suggest that orexin-A could play a pivotal role in stimulating glucose utilization in grouper, for a long-term goal, which might be useful in reducing costs in the aquaculture industry. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10956433
Volume :
199
Database :
Academic Search Index
Journal :
Comparative Biochemistry & Physiology Part A: Molecular & Integrative Physiology
Publication Type :
Academic Journal
Accession number :
117144954
Full Text :
https://doi.org/10.1016/j.cbpa.2016.05.027