PCT及CRP在急性肠梗阻大鼠血清中的表达水平及其临床意义.

Objective: To investigate the level and clinical significance of procalcitonin(PCT) and C-reactive protein(CRP) in plasma of rats with acute intestinal obstruction. Methods: 83 Wistar rats were randomly divided into control group(n=13), sham operation group(n=35) and acute intestinal obstruction group(n=35). The rats in control group were sacrificed, the rats in the sham group were only taken the open surgery, and the rats in the intestinal obstruction group were taken the open surgery and the ligation of the terminal ileum. The serum levels of CRP and PCT were detected at different time points, and the pathological changes of ileum in rats with acute intestinal obstruction were observed. Results: The level of PCT and CRP in sham operation group was gradually increased at 8h and 24 h and decreased gradually at 48 h, 72 h and 96 h, and the level of PCT was higher than that of control group at different time points, but the level of CRP at 96 h was returned to control. The level of PCT and CRP in intestinal obstruction group at different time points were significantly higher than those of the control group, and it was gradually increased to the peak at the end of the experiment,and the level of PCT and CRP in intestinal obstruction group at 48 h, 72 h and 96 h were significantly higher than those in sham operation group with statistical significance(P<0.05). Histopathological examination showed that the structure of the intestinal wall in the control group was normal, and mild pathological changes were detected in the sham operation group, and the ileum tissues of the intestinal obstruction group were obviously damaged, with the villi necrosis, severe edema and inflammatory cell infiltration. Conclusions: The levels of PCT and CRP can reflect the state of intestinal obstruction and the extent of intestinal mucosal damage. [ABSTRACT FROM AUTHOR]