Sinergismo en la respuesta inmune en ratones BALB/C//BIOULA con parasitosis combinadas: Syphacia obvelata y Leishmania mexicana.

Introduction: BALB/C//BIOULA mice are used in experiments studying parasites of the Mexican Leishmania species, determining IgG concentrations. Previous studies have shown that mice parasitized with Syphacia obvelata present high titers of IgG. The aim of this study was to determine if there is synergism in the immunological response of BALB/C//BIOULA mice with combined parasitism. Materials and methods: The animals were divided into four groups: Group A, without parasites; Group B, parasitized with Syphacia obvelata; Group C, with Mexican Leishmania; and Group D, with combined parasitism. Over two months, the mice were monitored using growth curves, coproparasitological diagnosis, and hematological and antibody titer evaluation through ELISA. Results: The growth curves indicated that mice with combined parasitism gained less weight and size, and possessed the highest quantity of Syphacia obvelata eggs and adult parasites. Statistically significant differences were found in the values of hematological parameters, and measurement of IgG concentration also showed that the highest quantity of IgG units occurred for sera of mice with combined parasitism. Conclusions: the results of the growth curves, the hematological tests and the IgG titers confirmed that there is a synergism in the response of rats parasitized with Syphacia obvelata and Mexican Leishmania. This may lead to false positive results when researchers conduct experiments with Mexican Leishmania on mice that are parasitized with Syphacia obvelata. [ABSTRACT FROM AUTHOR]