Back to Search
Start Over
Quantitative analysis of glycerol in dicarboxylic acid-rich cutins provides insights into Arabidopsis cutin structure.
- Source :
-
Phytochemistry . Oct2016, Vol. 130, p159-169. 11p. - Publication Year :
- 2016
-
Abstract
- Cutin is an extracellular lipid polymer that contributes to protective cuticle barrier functions against biotic and abiotic stresses in land plants. Glycerol has been reported as a component of cutin, contributing up to 14% by weight of total released monomers. Previous studies using partial hydrolysis of cuticle-enriched preparations established the presence of oligomers with glycerol-aliphatic ester links. Furthermore, glycerol-3-phosphate 2- O -acyltransferases ( sn -2-GPATs) are essential for cutin biosynthesis. However, precise roles of glycerol in cutin assembly and structure remain uncertain. Here, a stable isotope-dilution assay was developed for the quantitative analysis of glycerol by GC/MS of triacetin with simultaneous determination of aliphatic monomers. To provide clues about the role of glycerol in dicarboxylic acid (DCA)-rich cutins, this methodology was applied to compare wild-type (WT) Arabidopsis cutin with a series of mutants that are defective in cutin synthesis. The molar ratio of glycerol to total DCAs in WT cutins was 2:1. Even when allowing for a small additional contribution from hydroxy fatty acids, this is a substantially higher glycerol to aliphatic monomer ratio than previously reported for any cutin. Glycerol content was strongly reduced in both stem and leaf cutin from all Arabidopsis mutants analyzed ( gpat4 / gpat8 , att1-2 and lacs2-3 ). In addition, the molar reduction of glycerol was proportional to the molar reduction of total DCAs. These results suggest “glycerol-DCA-glycerol” may be the dominant motif in DCA-rich cutins. The ramifications and caveats for this hypothesis are presented. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GLYCERIN
*DICARBOXYLIC acids
*CUTIN
*ARABIDOPSIS
*POLYMER structure
*ABIOTIC stress
Subjects
Details
- Language :
- English
- ISSN :
- 00319422
- Volume :
- 130
- Database :
- Academic Search Index
- Journal :
- Phytochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 117709724
- Full Text :
- https://doi.org/10.1016/j.phytochem.2016.03.017