Back to Search
Start Over
Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography.
- Source :
-
Biomicrofluidics . 2016, Vol. 10 Issue 4, p1-9. 9p. - Publication Year :
- 2016
-
Abstract
- Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19321058
- Volume :
- 10
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biomicrofluidics
- Publication Type :
- Academic Journal
- Accession number :
- 117823803
- Full Text :
- https://doi.org/10.1063/1.4959239