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Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples.
- Source :
-
Food & Agricultural Immunology . Nov2016, Vol. 27 Issue 6, p830-840. 11p. - Publication Year :
- 2016
-
Abstract
- A specific monoclonal antibody (mAb) against trimethoprim (TMP) was produced and a mAb-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established to detect TMP residues in milk, honey, and fish samples. The working range of ic-ELISA and IC50was 1.83–9.36 and 4.14 µg L−1. The average recoveries of TMP from TMP-spiked milk, honey, and fish sample were 103.5–118.0%, 100.3–107.1%, and 91.5–108.1%, respectively. The results revealed that our developed mAb-based ic-ELISA can be effectively applied to detect TMP residues in these food products. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 27
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 117876935
- Full Text :
- https://doi.org/10.1080/09540105.2016.1183599