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Optimization of the fermentation process of Cordyceps sobolifera Se-CEPS and its anti-tumor activity in vivo.
- Source :
-
Journal of Biological Engineering . 6/23/2016, Vol. 10, p1-9. 9p. - Publication Year :
- 2016
-
Abstract
- Background: Cordyceps sobolifera (C. sobolifera) isolated from cicadae was used as the starting fungus to produce selenium-enriched C. sobolifera extracellular polysaccharide (Se-CEPS). An orthogonal experimental design based on a single-factor experiment was used to optimize the C. sobolifera fermentation conditions, including the potato juice, peptone, and KH2PO4 concentrations. Ultraviolet (UV) and infrared (IR) analyses of CEPS and Se-CEPS were conducted, as well as an in vivo anti-tumor analysis. Results: Under optimal conditions (i.e., 40 potato juice, 0.4 KH2PO4, and 0.5 % peptone), the fermentation yield of Se-CEPS was 5.64 g/L. UV and IR spectra showed that Se-CEPS contained a characteristic absorption peak of a selenite Se = O double bond, demonstrating the successful preparation of Se-CEPS. Activity tests showed that Se-CEPS improved the immune organ index, serum cytokine content, and CD8+ and CD4+ T lymphocyte ratio in colon cancer CT26 tumor-bearing mice, thereby inhibiting tumor growth. When combined with 5-FU, Se-CEPS reduced the toxicity and enhanced the function of 5-FU. Conclusion: The result of these experiments indicated that orthogonal experimental design is a promising method for the optimization of Se-CEPS production, and the Se-CEPS from C. sobolifera can improve the anti-tumor capacity of mice. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CORDYCEPS
*SELENIUM
*FERMENTATION
*TUMOR growth
*LABORATORY mice
*THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 17541611
- Volume :
- 10
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 117882854
- Full Text :
- https://doi.org/10.1186/s13036-016-0029-0