Back to Search
Start Over
Evaluation of a new extraction protocol for yeast identification by mass spectrometry.
- Source :
-
Journal of Microbiological Methods . Oct2016, Vol. 129, p61-65. 5p. - Publication Year :
- 2016
-
Abstract
- In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01677012
- Volume :
- 129
- Database :
- Academic Search Index
- Journal :
- Journal of Microbiological Methods
- Publication Type :
- Academic Journal
- Accession number :
- 117919966
- Full Text :
- https://doi.org/10.1016/j.mimet.2016.08.001