基于RNA干扰CMTM7 对肺腺癌A549细胞凋亡的影响.

Objective: To investigate the effects of RNA interference CMTM7 on apoptosis of lung adenocarcinoma A549 cell by the. Methods: Selected lung adenocarcinoma A549 cell were divided into si RNA group, negative control group and blank control group,and the RNA interference CMTM7 vectors were transfected into the logarithmic growth of A549 cells, observed A549 cell growth, and the apoptosis and cell cycle status was detected. Results: MTT experiment showed that the inhibition rate in si RNA transfection group was significantly higher than that of the negative control group and blank control group(P>0.05), while the difference between the negative control group and the control group were not significant(P<0.05). Flow cytometry showed the apoptosis rate in the si RNA transfection group was significantly higher than that of the negative control group and blank control group(P<0.05), while the difference between the negative control group and the control group were not significant(P>0.05). Flow cytometry experiments showed that the number of G0 / G1 phase cell in the si RNA transfection group was increased with significantly difference compared with blank control group and negative control group(P<0.05), while the number of G2 / M phase cell in the si RNA transfection group was less than that of the control group and negative control group(P>0.05), but it had no significant difference between the blank control group and negative control group(P>0.05). Conclusions: RNA interference CMTM7 can promote lung adenocarcinoma A549 cell apoptosis, inhibit the growth of tumor cells, and its mechanism may be achieved by interfering with the cell cycle. [ABSTRACT FROM AUTHOR]