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Chitosan-iron oxide nanocomposite based electrochemical aptasensor for determination of malathion.
- Source :
-
Analytica Chimica Acta . Oct2016, Vol. 939, p108-116. 9p. - Publication Year :
- 2016
-
Abstract
- An electrochemical aptasensor based on chitosan-iron oxide nanocomposite (CHIT-IO) film deposited on fluorine tin Oxide (FTO) was developed for the detection of malathion. Iron oxide nanoparticles were prepared by co-precipitation method and characterized by Transmission electron microscopy and UV–Visible spectroscopy. The biotinylated DNA aptamer sequence specific to the malathion was immobilized onto the iron oxide doped-chitosan/FTO electrode by using streptavidin as linking molecule. Various characterization studies like Field Emission-Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and Electrochemical studies were performed to attest the successful fabrication of bioelectrodes. Experimental parameters like aptamer concentration, response time, stability of electrode and reusability studies were optimized. Aptamer immobilized chitosan-iron oxide nanocomposite (APT/SA/CHIT-IO/FTO) bioelectrodes exhibited LOD of about 0.001 ng/mL within 15 min and spike-in studies revealed about 80–92% recovery of malathion from the lettuce leaves and soil sample. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHITOSAN
*IRON oxides
*NANOCOMPOSITE materials
*MALATHION
*FLUORINE
Subjects
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 939
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 118074331
- Full Text :
- https://doi.org/10.1016/j.aca.2016.08.015