Duplex PCR Detection of Pseudomonas savastanoi pv. phaseolicola and Curtobacterium flaccumfaciens pv. flaccumfaciens in Soybean.

The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola (Psp) and Curtobacterium flaccumfaciens pv. Flaccumfaciens (Cff). Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was developed using the combined primers PSPF1/PSPR2 and CffFl/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity and sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was confirmed in the artificially inoculated soybean samples imported. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp and Cff horn imported soybean. [ABSTRACT FROM AUTHOR]