广西普通油茶种质资源遗传多样性的SSR 分析.

Oil tea (Camellia oleifera) is the important oiltree in our country with the vastest area of distribution and the most products in the genus of Camellia. Guangxi Zhuang Autonomous Region, the important distribution area of oil tea, abounds in oil tea germplasm resources. In order to evaluate genetic variation of germplasm resources of oil tea in Guan-gxi for germplasm conservation and cultivar selection, 10 SSR primer pairs were screened and evaluated in the present study, then genetic diversity of 97 representive germplasm resources of oil tea in Guangxi was analysed based on the polymorphic SSR markers. The findings indicated as follows:Firstly, of the ten developed SSR primer pairs, seven codo-minant primer pairs could produced stable amplicons, and another two primers could produce unstable amplicons; the remaining one yielded no band on the gel. Secondly, a total of 33 alleles were detected among 97 oil tea germplasm re-sources using seven codominant SSRs, and the number of alleles was from three to six with an average of 4.714 3 for ev-ery pair of primers;the number of effective allele ranged from 2.084 2 to 4.314 8 with an average of 2.828 8, and the gene diversity was from 0.520 2 to 0.768 2 with an average of 0.628 1. Thirdly, almost loci out of Hardy-Weinberg equi-librium meant genetic structure in the population, the ranges of observed and expected heterozygosity were 0. 413 0-0.670 1 and 0.523 3-0.772 4, with the averages of 0.569 8 and 0.631 6. Finally, the range of genetic distances among germplasm resources was 0.05-0.791 7, with an average of 0.354 5. The cluster analysis based on UPGMA method re-vealed that the germplasm resources from the same places could not cluster in the same branch, on the other hand, the germplasm resources in one branch came from several places. The foregoing results reveavled that the developed SSRs of oil tea were suitable to work in the oil tea in Guangxi and the genetic diversity of germplasm resources in Guangxi was relatively higher. Those findings provide the scientific evidences for deep exploitation and efficient utilization of genetic resources of oil tea in Guangxi. [ABSTRACT FROM AUTHOR]