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Enhanced detoxification and degradation of herbicide atrazine by a group of O-methyltransferases in rice.
- Source :
-
Chemosphere . Dec2016, Vol. 165, p487-496. 10p. - Publication Year :
- 2016
-
Abstract
- Atrazine (ATR) as a toxic herbicide has become one of the seriously environmental contaminants worldwide due to its long-term intensive use in crop production. This study identified novel methyltransferases (MTs) involved in detoxification and degradation of ATR residues in rice plants. From a subset of MTs differentially expressed in ATR-exposed rice, forty-four O -methyltransferase genes were investigated. Total activities were significantly enhanced by ATR in rice tissues. To prove detoxifying capacity of the MTs in rice plants, two rice O -MTs (LOC_Os04g09604 and LOC_Os11g15040) were selected and transformed into yeast cells ( Pichia pastoris X-33). The positive transformants accumulated less ATR and showed less toxicity. Using UPLC-TOF-MS/MS, ATR-degraded products in rice and yeast cells were characterized. A novel O -methylated-modified metabolite (atraton) and six other ATR-derivatives were detected. The topological interaction between LOC_Os04g09604 enzyme and its substrate was specially analyzed by homology modeling programs, which was well confirmed by the molecular docking analysis. The significance of the study is to provide a better understanding of mechanisms for the specific detoxification and degradation of ATR residues in rice growing in environmentally relevant ATR-contaminated soils and may hold a potential engineering perspective for generating ATR-resistant rice that helps to minimize ATR residues in crops. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METABOLIC detoxification
*HERBICIDES
*ATRAZINE
*METHYLTRANSFERASES
*RICE
Subjects
Details
- Language :
- English
- ISSN :
- 00456535
- Volume :
- 165
- Database :
- Academic Search Index
- Journal :
- Chemosphere
- Publication Type :
- Academic Journal
- Accession number :
- 118542984
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2016.09.025