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Comparative analysis of l-sorbose dehydrogenase by docking strategy for 2-keto- l-gulonic acid production in Ketogulonicigenium vulgare and Bacillus endophyticus consortium.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . Nov2016, Vol. 43 Issue 11, p1507-1516. 10p. - Publication Year :
- 2016
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Abstract
- Improving the yield of 2-keto- l-gulonic acid (2-KGA), the direct precursor of vitamin C, draws more and more attention in industrial production. In this study, we try to increase the 2-KGA productivity by computer-aided selection of genes encoding l-sorbose dehydrogenases (SDH) of Ketogulonicigenium vulgare. First, six SDHs were modeled by docking strategy to predict the binding mode with co-factor PQQ. The binding energy between SSDA1-H/SSDA1-L and PQQ was the highest, followed by SSDA3/SSDA2. The binding energy between SSDA1-P/SSDB and PQQ was the lowest. Then, these genes were overexpressed, respectively, in an industrial strain K. vulgare HKv604. Overexpression of ssda1- l and ssda1- h enhanced the 2-KGA production by 7.89 and 12.56 % in mono-cultured K. vulgare, and by 13.21 and 16.86 % when K. vulgare was co-cultured with Bacillus endophyticus. When the engineered K. vulgare SyBE_Kv000116013 (overexpression of ssda1- p) or SyBE_Kv000116016 (overexpression of ssdb) was co-cultured with B. endophyticus, the 2-KGA production decreased significantly. The docking results were in accordance with the experimental data, which indicated that computer-aided modeling is an efficient strategy for screening more efficient enzymes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 43
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 118833634
- Full Text :
- https://doi.org/10.1007/s10295-016-1829-4