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l -carnitine protects rat hepatocytes from oxidative stress induced by T-2 toxin.
- Source :
-
Drug & Chemical Toxicology . Oct2016, Vol. 39 Issue 4, p445-450. 6p. - Publication Year :
- 2016
-
Abstract
- Context: Oxidative stress and mitochondrial dysfunction are thought to be the main mechanism of T-2 toxin toxicity. T-2 toxin is the most potent trichothecene mycotoxin which is present in agricultural products. L-carnitine, besides its anti-oxidative properties, facilitates the transportation of long-chain fatty acids in to mitochondrial matrix.Objective: In this study we tested whether L-carnitine, an antioxidant and a facilitator for long-chain fatty acid transportation across mitochondrial membranes, could protect rat hepatocytes against toxicity induced by T-2 toxin.Materials and methods: L-carnitine in low and high doses (50 and 500 mg/kg) was administered for five consecutive days to male Wistar rats. Hepatocytes were isolated and freshly exposed to appropriate concentration of T-2 toxin for 2 h followed by oxidative stress and cell death evaluations.Results: Glutathione depletion, ROS overproduction and mitochondrial membrane potential collapse were determined under T-2 toxin exposure. Pretreatment with L-carnitine particularly at high-dose reduced toxicity and prevented the hepatocytes from abnormal caspase-3 activity and apoptosis.Conclusion: Low toxicity of L-carnitine and its mitochondrial protective effects promises an effective way to reduce or prevent the toxicity induced by certain environmental pollutants, including T-2 toxin. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01480545
- Volume :
- 39
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Drug & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 118836046
- Full Text :
- https://doi.org/10.3109/01480545.2016.1141423